Enzymes lab report
Maria Castro
time (sec) tubes 2 and 3 4 ◦ c tubes 4 and 5 24 ◦ c tubes 6 and 7 32 ◦ c tubes 8 and 9 48 ◦ c 20 0.27 0.35 0.49 0.72 40 0.39 0.52 0.7 0.95 60 0.52 0.63 0.84 1.1 80 0.63 0.72 0.94 1.2
100 0.73 0.79 1.02 1.25 120 0.82 0.85 1.08 1.3
Temperature Effect on Peroxidase
A B SO
R B A N
C E
0
0.35
0.7
1.05
1.4
TIME (SEC)
20 40 60 80 100
y = 0.1114x + 0.6967
y = 0.1146x + 0.444
y = 0.0971x + 0.3033
y = 0.1109x + 0.172
tubes 2 and 3 4 ◦ c y = 0.1109x + 0.172 tubes 4 and 5 24 ◦ c tubes 6 and 7 32 ◦ c y = 0.1146x + 0.444 tubes 8 and 9 48 ◦ c
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Temperature Effect on Peroxidase
TIME (SEC)
100 120
tubes 2 and 3 4 ◦ c y = 0.1109x + 0.172 tubes 4 and 5 24 ◦ c y = 0.0971x + 0.3033 tubes 6 and 7 32 ◦ c y = 0.1146x + 0.444 tubes 8 and 9 48 ◦ c y = 0.1114x + 0.6967
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4 ◦ c 24 ◦ c RATE ( CHANGE OF ABSORBANCE/SECOND) 0.1109 0.0971
Slope of the Temperature Effect on Peroxidase
R AT
E (
C H
A N
G E
O F
A B SE
R B A N
C E/
SE
C O
N D
)
0.08
0.09
0.1
0.11
0.12
Temperature(◦ C)
4 ◦ c
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32 ◦ c 48 ◦ c 0.1146 0.1114
Slope of the Temperature Effect on Peroxidase
Temperature(◦ C)
24 ◦ c 32 ◦ c 48 ◦ c
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TIME (SEC) TUBES 2 AND 3 PH 3 TUBES 4 AND 5 PH 5 TUBES 6 AND 7 PH 7 TUBES 8 AND 9 PH 9 20 0.31 0.63 0.41 0.17 40 0.36 1.01 0.59 0.19 60 0.42 1.29 0.73 0.21 80 0.46 1.51 0.84 0.23
100 0.5 1.69 0.92 0.23 120 0.53 1.85 1 0.24
EFFECTS OF PH ON PEROXIDASE ACTIVITY
A B SO
R B A N
C E
0
0.5
1
1.5
2
TIME (SEC)
20 40
y = 0.014x + 0.1627
y = 0.1157x + 0.3433
y = 0.2389x + 0.494
y = 0.0446x + 0.274
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EFFECTS OF PH ON PEROXIDASE ACTIVITY
TIME (SEC)
40 60 80 100 120TUBES 2 AND 3 PH 3 y = 0.0446x + 0.274 TUBES 4 AND 5 PH 5 y = 0.2389x + 0.494 TUBES 6 AND 7 PH 7 y = 0.1157x + 0.3433 TUBES 8 AND 9 PH 9 y = 0.014x + 0.1627
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