Micro-Unknown Research
CarlaB
Report Form for Gram-Positive Unknown Unknown ID Code:
MORPHOLOGICAL FEATURES (observations and results):
*Gram Stain Reaction:______________________________________________________
*Cell Morphology and Arrangement:___________________________________________
Acid-fast Stain: ___________________________________________________________
Capsular Stain: ___________________________________________________________
*CULTURAL CHARACTERISTICS (observation of colonies on TSA and original unknown broth)
Cultural characteristics in broth: ______________________________
Colony Form:_____________________________________________
Colony Size in mm: ________________________________________
Colony Margin:____________________________________________
Colony Texture____________________________________________
Colony Elevation:__________________________________________
Colony Surface Appearance:__________________________________
Colony Pigment:_(+ or -)___________ Color:_________ H2O soluble?________
PHYSIOLOGICAL CHARACTERISTICS: For lines that just have blanks, include observations and results
*Catalase:________________________________________________
*Glucose Fermentation Broth:_______________________________________________
*Lactose Fermentation Broth:________________________________________________
*Sucrose Fermentation Broth:________________________________________________
*Mannitol Fermentation Broth:_______________________________________________
* Nitrate Reduction ( + or -) :______; observation after addition of:
reagent A&B(color):____________ zinc (color):_____________
*Hemolysis (circle one): + or - ; ________________; ________________ ; ________________
Motility:_____________________
Mannitol salt agar: 7.5% Salt tolerance: _________________________________________________
Mannitol fermentation: _______________________________________________
Coagulase: _________________________________________________
Bile esculin: ________________________________________________
Bacitracin sensitivity: Results:_____________ Zone size (in mm) Observed: _____________
Optochin sensitivity: Results: ______________ Zone size (in mm) Observed: _____________
Suspected Unknown: ______________________________________________________
*Required Tests. All other tests are follow-up tests that depend on the results of the required tests. Be sure that you have carried out all of the appropriate follow-up tests that pertain to your organism (see pg 296 in the lab manual).
- *Gram Stain Reaction:______________________________________________________
- *Cell Morphology and Arrangement:___________________________________________
- *CULTURAL CHARACTERISTICS (observation of colonies on TSA and original unknown broth)
- Cultural characteristics in broth: ______________________________
- Colony Form:_____________________________________________
- Colony Size in mm: ________________________________________
- Colony Margin:____________________________________________
- Colony Texture____________________________________________
- Colony Elevation:__________________________________________
- Colony Surface Appearance:__________________________________
- Colony Pigment:_(+ or -)___________ Color:_________ H2O soluble?________
- *Catalase:________________________________________________