Micro-Unknown Research

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Report Form for Gram-Positive Unknown Unknown ID Code:

MORPHOLOGICAL FEATURES (observations and results):

*Gram Stain Reaction:______________________________________________________

*Cell Morphology and Arrangement:___________________________________________

Acid-fast Stain: ___________________________________________________________

Capsular Stain: ___________________________________________________________

*CULTURAL CHARACTERISTICS (observation of colonies on TSA and original unknown broth)

Cultural characteristics in broth: ______________________________

Colony Form:_____________________________________________

Colony Size in mm: ________________________________________

Colony Margin:____________________________________________

Colony Texture____________________________________________

Colony Elevation:__________________________________________

Colony Surface Appearance:__________________________________

Colony Pigment:_(+ or -)___________ Color:_________ H2O soluble?________

PHYSIOLOGICAL CHARACTERISTICS: For lines that just have blanks, include observations and results

*Catalase:________________________________________________

*Glucose Fermentation Broth:_______________________________________________

*Lactose Fermentation Broth:________________________________________________

*Sucrose Fermentation Broth:________________________________________________

*Mannitol Fermentation Broth:_______________________________________________

* Nitrate Reduction ( + or -) :______; observation after addition of:

reagent A&B(color):____________ zinc (color):_____________

*Hemolysis (circle one): + or - ; ________________; ________________ ; ________________

Motility:_____________________

Mannitol salt agar: 7.5% Salt tolerance: _________________________________________________

Mannitol fermentation: _______________________________________________

Coagulase: _________________________________________________

Bile esculin: ________________________________________________

Bacitracin sensitivity: Results:_____________ Zone size (in mm) Observed: _____________

Optochin sensitivity: Results: ______________ Zone size (in mm) Observed: _____________

Suspected Unknown: ______________________________________________________

*Required Tests. All other tests are follow-up tests that depend on the results of the required tests. Be sure that you have carried out all of the appropriate follow-up tests that pertain to your organism (see pg 296 in the lab manual).

  • *Gram Stain Reaction:______________________________________________________
  • *Cell Morphology and Arrangement:___________________________________________
    • *CULTURAL CHARACTERISTICS (observation of colonies on TSA and original unknown broth)
  • Cultural characteristics in broth: ______________________________
  • Colony Form:_____________________________________________
  • Colony Size in mm: ________________________________________
  • Colony Margin:____________________________________________
  • Colony Texture____________________________________________
  • Colony Elevation:__________________________________________
  • Colony Surface Appearance:__________________________________
  • Colony Pigment:_(+ or -)___________ Color:_________ H2O soluble?________
  • *Catalase:________________________________________________